简易分光光度计分析桧木纯露成分

摘要：从桧木萃取精油后的废料中，可萃取出桧木纯露，具有良好的杀菌效果，但由于桧木纯露的品管与浓度量测成本 较高，限制了其应用。本研究尝试寻找适当的光谱频率，以分光光度计所侦测到的吸光度作为桧木纯露浓度的指标。本 研究采用气相色谱-质谱法（（ GC-MS）分析桧木纯露的成分。在确定成分后，将桧木纯露原液以纯水稀释至100、50、25、 0％v/v，再以简易分光光度计对测试样品进行测试。经简易分光光度计进行光谱检测后，确认波长介于 205–350nm之间 的光谱最具代表性。吸光度与纯露浓度大致呈线性关系，这表明可以使用简单的分光光度计开发一种低成本、高灵敏度 的测定方法。

关键词：柏木纯露；GC-MS；分光光度计；浓度测量

期刊：Mathematical Biosciences and Engineering

DOI：10.3934/mbe.2021445

设备：GaiaField

Using a simple spectrophotometer to analyze cypress hydrolat composition

Abstract：The Pure Dew (Cypress Hydrolat), which could be extracted from the waste material after the extracting essential oil from Taiwan cypress, has a good bactericidal effect. However, due to the high cost on quality control and concentration measurement of the Pure Dew, its application was restricted. This research tries to find suitable spectral frequencies through which the absorbance detected by the spectrometer could be used as the index of the pure dew concentration. This study used Gas Chromatography-Mass Spectrophotometer (GC-MS) to analyze the composition of Taiwan cypress hydrolat. After obtaining the composition, the raw liquor of cypress hydrolat was diluted to 100, 50, 25 and 0% v/v with pure water. The test samples were then tested by a simple spectrophotometer. After the spectrographic detection of absorbance using a simple spectrophotometer, it is confirmed that the spectrum of wavelength between 205–350 nm is the most representative. The absorptance and the pure dew concentration was roughly in linear relation which suggested that a simple spectrophotometer can be used to develop a low-cost and high.

Keywords：cypress hydrolat; GC-MS; spectrophotometer; concentration measurement